Description
The assay is designed for diagnostic laboratory use (not self‑testing). Glucose‑6‑phosphate dehydrogenase in the sample oxidizes glucose‑6‑phosphate to 6-phosphogluconate while reducing NADP to NADPH. NADPH is then quantified (colorimetrically using tetrazolium salt or via fluorescence in a variant). The absorbance or fluorescence signal is proportional to enzyme activity.
G6PD deficiency is a common enzymatic disorder affecting red blood cell antioxidant pathways. In deficiency, red cells are susceptible to oxidative damage, leading to hemolysis especially under stress (e.g. infection, certain drugs, fava beans). Clinical presentation may range from asymptomatic to neonatal jaundice or chronic hemolysis.
The assay offers a simplified one‑step protocol with results in under 1 hour for the fluorescent version. It supports both manual and automated workflows, with no transfer steps required. Packaging options and calibration curves on blotting paper are included.
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