Hemoglobinopathies

The principle of the Targeted MS/MS Hemo screening assay is based on a rapid hemoglobin (Hb) extraction and denaturation from patients’ DBS samples before its digestion by trypsin. The digested extract is then analysed by MS/MS, in flow injection analysis mode (FIA), on a “triple quadrupole” system. The MS/MS mode used for this assay is the Multiple Reaction Monitoring (MRM): two product ions are selected by peptide and the twelve peptides are simultaneously detected by scanning the two specific “parent ion/product ion couple” for each.

A dedicated software allows time saving in results analysis. Targeted MS/MS Hemo Software (based on Excel) is used to calculate peptide signals ratios, which are analysed by tree-like algorithms. It facilitates the identification of different medically significant phenotypes.